Large Airway Bronchial Wash Lipidomics as Novel Biomarkers for Chronic Lung Allograft Dysfunction
نویسندگان
چکیده
PurposeChronic lung allograft dysfunction (CLAD) is the primary cause of long-term mortality after transplantation. Aspiration bile acids has been associated with CLAD and lipidomic dysregulation. This study evaluates role large airway bronchial wash (LABW) lipidomics diagnosis CLAD.MethodsLABW (n=611) samples were obtained from 305 transplant recipients at different timepoints. Concentrations 26 specific lipid subfamilies using liquid chromatography mass spectrometry. Lipid percentages where stratified by highest tertiles. The outcome interest was development sampling time. Time-to-event analysis done Kaplan-Meier functions a multivariable Cox proportional hazards model adjusting for baseline comorbidities.ResultsSamples time collection showed higher hexosylceramides (HEXCER), CE, sphingomyelin (SM) lactosylceramide (LACCER). A high percentage bismethyl-phosphatidic acid (BMP), phosphatidylethanolamine (PEP), dehydrosphingosine (DHSM), lysophosphatidylethanolamine (LPE), ganglioside (GM3), phosphatidyl-serine (PS) an increased univariable analysis. CE (HR: 1.48, 95% CI: 1.09-2.03, P=0.013) independent predictors High CER 0.49, 0.36-0.66, P<0.001) LACCER:CER ratio 0.45, 0.34-0.62, independently decreased CLAD.ConclusionAirway qualititative changes show distinct phenotype that associate presence risk CLAD. Percentages ratios correlate Changes in LABW lipids may serve as biomarkers elucidate pathophysiologic transplant. Chronic comorbidities. Samples Airway
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ژورنال
عنوان ژورنال: Journal of Heart and Lung Transplantation
سال: 2022
ISSN: ['1053-2498', '1557-3117']
DOI: https://doi.org/10.1016/j.healun.2022.01.244